Research |
Research topics |
Enzymology.
Enzyme kinetics. Transient phase and steady-state. Rapid equilibrium.
Analytical
and numerical resolution of mechanisms. Mathematical modellization. Computer
simulation.
Enzyme
extraction. Differential centrifugation.
Mechanism of Polyphenol oxidase (EC 1.14.18.1). Mono- and diphenolase activities. Effect of inhibitors. Latency and kinetic cooperativity. Enzymatic synthesis of o-diphenols.
Enzymatic
inhibition (classical inhibitors, time dependent inhibitors, unstable
inhibitors, tight-binding inhibitors).
Mechanisms
of enzymatically catalyzed reactions involving one or more unstable species.
Zymogen
activation processes. Kinetics of the reaction in the presence of a chromogenic
substrate. Effect of different inhibitors. Pepsinogen, plasminogen,
trypsinogen, etc.
Biological
systems of cascade amplification.
Substrate
cycles. Analytical and clinical applications. Economical optimization. Amplification
of an enzymatic activity.
Glutathione-ascorbate
redox cycle in chloroplasts. Chemical and enzymatic reactions involved. Oxidative
stress.
Oxidoreductase
enzymes: Polyphenol oxidase, Peroxidase and Laccase.
Residence
time of an enzymatic system.
Research grants: Founded projects as
main researcher |
Kinetic study of biocatalytic processes using oxidoreductase enzymes. Founded
by Junta de Comunidades de Castilla-La Mancha, PAI08-0175-8618. 2008-2010.
Kinetic analysis of several biological mechanisms of response amplification against a signal. Founded by Junta de Comunidades de Castilla-La Mancha, PAI05-036. 2005-2007.